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Mapping Genome-wide Accessible Chromatin in Primary Human T Lymphocytes by ATAC-Seq
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JoVE Journal Genetica
Mapping Genome-wide Accessible Chromatin in Primary Human T Lymphocytes by ATAC-Seq

Mapping Genome-wide Accessible Chromatin in Primary Human T Lymphocytes by ATAC-Seq

DOI:
10.3791/56313-v

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09:08 min

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November 13, 2017

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, , , ,
1The Mina and Everard Goodman Faculty of Life Sciences,Bar-Ilan University

Capitoli

  • 00:05Titolo
  • 00:54Purification of Naïve Human CD4+ Cells, Polarization to Th1 and TH2 Cells, and Nuclei Extraction
  • 03:18Transposition Reaction and Library Amplification
  • 04:33Size Selection of ATAC-seq Libraries
  • 06:48Results: Quality Control and Next Generation Sequencing Reads
  • 08:07Conclusion

Summary

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Assay for Transposase-Accessible Chromatin coupled with high-throughput sequencing (ATAC-seq) is a genome-wide method to uncover accessible chromatin. This is a step-by-step ATAC-seq protocol, from molecular to the final computational analysis, optimized for human lymphocytes (Th1/Th2). This protocol can be adopted by researchers without prior experience in next-generation sequencing methods.

Tags

ATAC-SeqChromatin AccessibilityGenome-wideRegulatory ElementsT LymphocytesEpigenomicsPrimary CellsTransposaseNuclei IsolationTh1Th2Cell Activation

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