/

Ricerca sul cancro

/

线粒体膜电位检测 CLIC4 击倒诱导 HN4 细胞凋亡的实验研究

JoVE Journal
Ricerca sul cancro

È necessario avere un abbonamento a JoVE per visualizzare questo. Accedi o inizia la tua prova gratuita.

JoVE Journal Ricerca sul cancro

Detection of Mitochondria Membrane Potential to Study CLIC4 Knockdown-induced HN4 Cell Apoptosis In Vitro

Please note that all translations are automatically generated. Click here for the English version.

线粒体膜电位检测 CLIC4 击倒诱导 HN4 细胞凋亡的实验研究

DOI:

10.3791/56317-v

•

13:20 min

•

July 17, 2018

•

Jinsen Lu, Lele Wu, Xiaoke Wang, Jinhang Zhu, Juan Du, Bing Shen

¹School of Basic Medical Sciences,Anhui Medical University, ²Department of Orthopedics, Anhui Provincial Hospital,Anhui Medical University

Capitoli

00:04Titolo
00:33Cell Transfection
02:08Rh123 Labeling
04:21Detection of the Mitochondrial Membrane Potential (MMP)
06:29Confocal Laser Scanning Fluorescence Microscope
08:34Statistical Analysis
09:48Results: Detection of Mitochondrial Membrane Potential via Normal Fluorescent Microscopy and Confocal Laser Scanning Microscopy
11:53Conclusion

Summary

Traduzione automatica

在这里, 我们提出了一个详细的协议, 应用罗丹明123识别线粒体膜电位 (基质) 和研究 CLIC4 击倒诱导 HN4 细胞凋亡的体外。在普通荧光显微镜和共聚焦激光扫描荧光显微镜下, 记录了基质金属蛋白酶的实时变化。

Tags

Keywords: Mitochondria Membrane Potential CLIC4 Knockdown HN4 Cell Apoptosis Rhodamine-123 Polylysine Trypsin Cell Culture Transfection SiRNA

Article

Embed

AGGIUNGI ALLA PLAYLIST

Usage Statistics

Video correlati

如何学习基底膜刚度生物物理触发在前列腺癌和其他与年龄相关的病理或代谢性疾病

细胞膜延伸特性及其在癌细胞黏附动力学中的作用研究

细胞凋亡逆转后新形成乳腺癌干细胞样细胞的流式细胞仪检测

cd8⁺ t 细胞诱导体外肿瘤细胞凋亡的实时检测--研究肿瘤浸润髓内细胞的免疫抑制作用

将操纵的肿瘤相关中性粒细胞转移到肿瘤携带小鼠中,以研究其血管生成潜力在Vivo

在Vivo免疫荧光定位中用于评估癌症研究中的治疗和诊断抗体生物分布

体外测定研究肿瘤-巨噬菌体相互作用

细胞周期特异性测量βH2AX和细胞凋亡后热源毒性应力通过流细胞测定

利用活体中鸡胆膜研究妇科和泌尿癌

人类乳腺上皮细胞致癌转化的体外评价

Read Article