Rivista
/
Ricerca sul cancro
/
Generation of Prostate Cancer Cell Models of Resistance to the Anti-mitotic Agent Docetaxel
JoVE Journal
Ricerca sul cancro
This content is Free Access.
JoVE Journal Ricerca sul cancro
Generation of Prostate Cancer Cell Models of Resistance to the Anti-mitotic Agent Docetaxel

Generation of Prostate Cancer Cell Models of Resistance to the Anti-mitotic Agent Docetaxel

DOI:
10.3791/56327-v

•

06:44 min

•

September 08, 2017

•

, , , , ,
1Department of Pathology, Tisch Cancer Institute,Icahn School of Medicine at Mount Sinai, 2Department of Oncological Sciences, Tisch Cancer Institute,Icahn School of Medicine at Mount Sinai

Capitoli

  • 00:05Titolo
  • 00:52Generation of Docetaxel-resistant Prostate Cancer Cells
  • 03:33Functional Characterization of Acquired Docetaxel Resistance by Colony Formation Assay
  • 05:10Results: Generation of Docetaxel-resistant Prostate Cancer Cell Model Systems and Functional Characterization of Docetaxel Resistance
  • 06:14Conclusion

Summary

Traduzione automatica

Traduzione automatica

Resistance to cancer therapies contributes to disease progression and death. Determining the mechanistic underpinnings of resistance is crucial for improving therapeutic response. This manuscript details the protocol to generate taxane-resistant cell models of prostate cancer (PC) to help dissecting the pathways involved in progression to Docetaxel resistance in PC patients.

Tags

Docetaxel-resistant Prostate Cancer Cell ModelsCancer Cell ResistanceAnti-mitotic Therapy ResistanceDrug Response ModelsCell Line DevelopmentDU145 Cells22Rv1 CellsDose EscalationCell CultureTrypsinizationCell PassagingPooled Resistant Cells

Article

Embed

AGGIUNGI ALLA PLAYLIST

Usage Statistics

Video correlati

Using RNA-sequencing to Detect Novel Splice Variants Related to Drug Resistance in In Vitro Cancer Models

Intracarotid Cancer Cell Injection to Produce Mouse Models of Brain Metastasis

Looking for Driver Pathways of Acquired Resistance to Targeted Therapy: Drug Resistant Subclone Generation and Sensitivity Restoring by Gene Knock-down

Virus Delivery of CRISPR Guides to the Murine Prostate for Gene Alteration

Identification, Histological Characterization, and Dissection of Mouse Prostate Lobes for In Vitro 3D Spheroid Culture Models

Establishing Cell Lines Overexpressing DR3 to Assess the Apoptotic Response to Anti-mitotic Therapeutics

Establishment of Gastric Cancer Patient-derived Xenograft Models and Primary Cell Lines

Using Human Induced Pluripotent Stem Cells for the Generation of Tumor Antigen-specific T Cells

Evaluating the Differentiation Capacity of Mouse Prostate Epithelial Cells Using Organoid Culture

Spatial Profiling of Protein and RNA Expression in Tissue: An Approach to Fine-Tune Virtual Microdissection

Read Article