Rivista
/
Ricerca sul cancro
/
应用肺转移法研究骨肉瘤转移性肺定植的实用思考
JoVE Journal
Ricerca sul cancro
È necessario avere un abbonamento a JoVE per visualizzare questo.  Accedi o inizia la tua prova gratuita.
JoVE Journal Ricerca sul cancro
Practical Considerations in Studying Metastatic Lung Colonization in Osteosarcoma Using the Pulmonary Metastasis Assay
Please note that all translations are automatically generated. Click here for the English version.

应用肺转移法研究骨肉瘤转移性肺定植的实用思考

DOI:
10.3791/56332-v

•

07:44 min

•

March 12, 2018

•

,
1Pediatric Oncology Branch, Center for Cancer Research, National Cancer Institute,National Institutes of Health, 2BC Cancer Agency,Provincial Health Services Authority, 3Department of Pathology and Laboratory Medicine,University of British Columbia

Capitoli

  • 00:04Titolo
  • 00:52Tail Vein Injection and Lung Insufflation
  • 02:48Widefield Fluorescence Lung Slice Imaging and Analysis
  • 05:49Results: Representative Lung Slice Imaging Analysis
  • 06:44Conclusion

Summary

Traduzione automatica

Traduzione automatica

本文的目的是为肺转移试验 (美洲狮) 的协议提供详细的描述。该模型允许研究人员使用 widefield 荧光或共聚焦激光扫描显微镜研究肺组织中的转移性骨肉瘤 (OS) 细胞生长。

Tags

Metastatic Lung ColonizationOsteosarcomaPulmonary Metastasis AssayTrachea CannulationAgarose InsufflationThree-dimensional MicroenvironmentTumor Cell VisualizationLung SlicesEx Vivo Culture

Article

Embed

AGGIUNGI ALLA PLAYLIST

Usage Statistics

Video correlati

肝大肠癌转移的高级动物模型：成像技术和转移性克隆性质

肺定植法在肺部组织循环肿瘤细胞可视化中的建立

应用 Fraumeni 综合征诱导多潜能干细胞建模骨肉瘤

脱细胞和细胞肺模型研究肿瘤转移

循环肿瘤细胞的微操作, 用于下游分子分析和转移电位评估

结合使用尾静脉转移分析与实时 Vivo 成像，以量化乳腺癌转移殖民化和肺负担

肿瘤细胞外侧尾静脉注射后肺转移的病理分析

利用计算机图像分析提高4T1乳腺癌模型中肺转移的定量

同源原位骨肉瘤Sprague Dawley大鼠模型，截肢控制转移率

研究癌症转移至肺部的永久窗口

Read Article