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肿瘤细胞系基因表达的条件性击倒研究单核/巨噬细胞在微环境中的招募

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Conditional Knockdown of Gene Expression in Cancer Cell Lines to Study the Recruitment of Monocytes/Macrophages to the Tumor Microenvironment

Please note that all translations are automatically generated. Click here for the English version.

肿瘤细胞系基因表达的条件性击倒研究单核/巨噬细胞在微环境中的招募

DOI:

10.3791/56333-v

•

10:59 min

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November 23, 2017

•

Marta H. Kubala², Yves A. DeClerck^2,3

¹Division of Hematology, Oncology and Blood and Marrow Transplantation, Department of Pediatrics,University of Southern California, ²The Saban Research Institute of Children’s Hospital Los Angeles, ³Department of Biochemistry and Molecular Medicine,University of Southern California

Capitoli

00:05Titolo
01:09Generation of Lentivirus Particles
02:34Generation of Stably Transfected Lines
04:55Isolation of Monocytes from Human Blood
08:00Boyden Chamber Migration Assay
09:25Results: PAI-1 Promotes Macrophage Migration
10:24Conclusion

Summary

Traduzione automatica

本议定书作为一个计划, 建立一个功能性的癌症细胞系的系统, 并随后使用, 特别是研究肿瘤细胞源性蛋白质在招募单核/巨噬细胞到肿瘤微环境的作用。

Tags

Keywords: Conditional Gene Knockdown Cancer Cell Lines Monocyte Recruitment Macrophage Recruitment Tumor Microenvironment Lentiviral Transduction HEK-293 Cells HCT-116 Cells MDA-MB-231 Cells Monocyte Purification Puromycin Selection

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