Rivista
/
/
Quantifying Microorganisms at Low Concentrations Using Digital Holographic Microscopy (DHM)
JoVE Journal
Bioingegneria
È necessario avere un abbonamento a JoVE per visualizzare questo.  Accedi o inizia la tua prova gratuita.
JoVE Journal Bioingegneria
Quantifying Microorganisms at Low Concentrations Using Digital Holographic Microscopy (DHM)
DOI:

07:27 min

November 01, 2017

, , , ,

Capitoli

  • 00:05Titolo
  • 00:56Growth and Enumeration of Bacteria
  • 02:38Preparation of Highly Dilute Samples for DHM and Recording DHM Videos
  • 04:06Calculations of Cell Density and Limits of Detection
  • 05:22Results: Growth Curves and Representative DHM Data
  • 06:14Conclusion

Summary

Traduzione automatica

Digital holographic microscopy (DHM) is a volumetric technique that allows imaging samples 50-100X thicker than brightfield microscopy at comparable resolution, with focusing performed post-processing. Here DHM is used for identifying, counting, and tracking microorganisms at very low densities and compared with optical density measurements, plate count, and direct count.

Video correlati

Read Article