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Mass Isolation and In Vitro Cultivation of Intramolluscan Stages of the Human Blood Fluke Schistosoma Mansoni
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Biologia dello sviluppo
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JoVE Journal Biologia dello sviluppo
Mass Isolation and In Vitro Cultivation of Intramolluscan Stages of the Human Blood Fluke Schistosoma Mansoni

Mass Isolation and In Vitro Cultivation of Intramolluscan Stages of the Human Blood Fluke Schistosoma Mansoni

DOI:
10.3791/56345-v

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12:05 min

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January 14, 2018

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, ,
1Department of Pathobiological Sciences,University of Wisconsin, School of Veterinary Medicine

Capitoli

  • 00:00Titolo
  • 03:18Processing of infected livers
  • 05:05Liver extraction and miracidial isolation
  • 08:06Miracidial harvesting and cultivation
  • 10:14Risultati
  • 10:49Conclusion

Summary

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This article describes a protocol for the large-scale axenic isolation and collection of free-swimming miracidia of the human blood fluke Schistosoma mansoni and their subsequent processing for introduction into in vitro culture.

Tags

Schistosoma MansoniMiracidiaIn Vitro CultivationMass IsolationSnail Intermediate HostNeglected Tropical DiseaseSchistosomiasisMouse ModelLiverEgg ExtractionSaline WashingArtificial Pond WaterLight AttractionLarval Cultivation

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