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Differentiering af musen embryonale stamceller i kortikale Interneuron prækursorer
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Neuroscienze
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JoVE Journal Neuroscienze
Differentiation of Mouse Embryonic Stem Cells into Cortical Interneuron Precursors

Differentiering af musen embryonale stamceller i kortikale Interneuron prækursorer

DOI:
10.3791/56358-v

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10:24 min

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December 03, 2017

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,
1Neuroscience Graduate Group, Perelman School of Medicine,University of Pennsylvania, 2Department of Psychiatry, Children’s Hospital of Philadelphia,University of Pennsylvania School of Medicine

Capitoli

  • 00:05Titolo
  • 00:44Culturing mESCs
  • 02:14Differentiation mESCs Toward MGE-like Telencephalic Progenitors
  • 04:57Somatostatin-enriching Protocol: “DD12 GFP High Sonic Hedgehog (SHH)”
  • 06:36PV-enriching Protocol: “DD11/DD17 mCherry + aPKCi”
  • 07:53Generation of Nkx2.1::mCherry and Lhx6::GFP Interneuron Precursors
  • 09:11Conclusion

Summary

Traduzione automatica

Traduzione automatica

Denne protokol beskriver en metode til at generere kortikale interneuron ophav og post mitotiske interneuron prækursorer fra mus embryonale stamceller ved hjælp af en modificeret embryoid krop til éncellelag metode. Disse stamfaderen/prækursorer kan være brugt i vitro fluorescently sorteret og transplanteret ind i neonatal neocortex for at studere skæbne bestemmelse eller anvendes til terapeutiske formål.

Tags

Mouse Embryonic Stem CellsCortical Interneuron ProgenitorsNkx2.1ParvalbuminSomatostatinMEF Feeder CellsTrypsin-EDTAGelatin-coated PlatesKSR-N2 MediaEmbryoid Bodies

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