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Purification of Viral DNA for the Identification of Associated Viral and Cellular Proteins
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Immunologia e infezioni
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JoVE Journal Immunologia e infezioni
Purification of Viral DNA for the Identification of Associated Viral and Cellular Proteins

Purification of Viral DNA for the Identification of Associated Viral and Cellular Proteins

DOI:
10.3791/56374-v

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08:26 min

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August 31, 2017

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,
1Department of Microbiology and Molecular Genetics,University of Pittsburgh School of Medicine

Capitoli

  • 00:05Titolo
  • 00:37Viral Infection and EdC Labeling
  • 01:57Purification of Viral DNA and Associated Proteins
  • 06:21Results: Protein Purification and Proteomics Data
  • 07:36Conclusion

Summary

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The goal of this protocol is to specifically tag and selectively isolate viral DNA from infected cells for the characterization of viral genome associated proteins.

Tags

Keywords: Viral DNA PurificationHerpes Simplex Virus Type 1Proteomic AnalysisCellular FactorsViral GenomeMRC-5 CellsHSV-1 InfectionViral DNA ReplicationEdC LabelingNuclei ExtractionNuclear PelletClick ReactionCellular Proteins

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