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病毒 DNA 的纯化及相关病毒和细胞蛋白的鉴定
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Immunologia e infezioni
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JoVE Journal Immunologia e infezioni
Purification of Viral DNA for the Identification of Associated Viral and Cellular Proteins
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病毒 DNA 的纯化及相关病毒和细胞蛋白的鉴定

DOI:
10.3791/56374-v

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08:26 min

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August 31, 2017

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,
1Department of Microbiology and Molecular Genetics,University of Pittsburgh School of Medicine

Capitoli

  • 00:05Titolo
  • 00:37Viral Infection and EdC Labeling
  • 01:57Purification of Viral DNA and Associated Proteins
  • 06:21Results: Protein Purification and Proteomics Data
  • 07:36Conclusion

Summary

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该协议的目的是专门标记和有选择地分离病毒 DNA 从受感染的细胞, 以表征病毒基因组相关的蛋白质。

Tags

Viral DNA PurificationHerpes Simplex Virus Type 1Proteomic AnalysisCellular FactorsViral GenomeMRC-5 CellsHSV-1 InfectionViral DNA ReplicationEdC LabelingNuclei ExtractionNuclear PelletClick ReactionCellular Proteins

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