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Whole-cell Currents Induced by Puff Application of GABA in Brain Slices
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Neuroscienze
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JoVE Journal Neuroscienze
Whole-cell Currents Induced by Puff Application of GABA in Brain Slices

Whole-cell Currents Induced by Puff Application of GABA in Brain Slices

DOI:
10.3791/56387-v

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07:32 min

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October 12, 2017

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, , ,
1School of Psychology,South China Normal University, 2School of Life Sciences,South China Normal University, 3Brain Science Institute,South China Normal University, 4Center for Studies of Psychological Application,South China Normal University, 5Guangdong Key Laboratory of Mental Health and Cognitive Science,South China Normal University

Capitoli

  • 00:05Titolo
  • 00:54Preparation of Prefrontal Cortex Slice
  • 02:57Whole-cell Patch Recording
  • 05:57Results: Dose and Puff-duration Response Curves
  • 06:30Conclusion

Summary

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We describe the puff technique, by which pharmacological reagents can be administered during whole-cell patch-clamp recording, and highlight various aspects of the features that are crucial for its success.

Tags

Whole-cell CurrentsGABABrain SlicesPuff ApplicationElectrophysiologyPharmacological AdministrationAcute Prefrontal Cortex SliceVibratomeACSFRecording ElectrodesPuff MicropipettesSodium Channel Blocker

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