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1Clinical Research Division,Fred Hutchinson Cancer Research Center, 2Hematology Branch, National Heart, Lung and Blood Institute,National Institutes of Health, 3Epigenetics Program, Department of Cell and Developmental Biology,University of Pennsylvania Perelman School of Medicine, 4Departments of Medicine and Biochemistry,University of Washington
Capitoli
- 00:04Titolo
- 01:17Selecting Single Cell Clones and Characterizing MeCP2 Reporter Expression
- 04:31Screening the Library for Reactivation Using Hygromycin B Selection
- 06:44Validating the Identified Hairpins ( Hits )
- 09:27Results: Isolation of Clones with the Reporter on the Inactive X Chromosome and Characterization of Hygromycin Resistance
- 10:17Conclusion
Divulgamos una pequeña horquilla RNA (shRNA) y el siguiente protocolo basado en la secuencia de generación para la identificación de reguladores de la inactivación del cromosoma x en una línea celular murina con luciferasa de luciérnaga y genes de resistencia a Higromicina fusionado a la metil CpG Unión proteína 2 ( MeCP2) gen en el cromosoma X inactivo.
