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Capitoli
- 00:08Introduction
- 00:27CellChip preparation: Hydrophilization, coating, incubation
- 03:41Bioreactor Setup
- 07:43Incubation Setup
- 08:58Analysis of organotypic functions
We describe a chip-based platform for the three-dimensional cultivation of cells in micro-bioreactors. One chip can house up to 10 Mio. cells that can be cultivated under precisely defined conditions with regard to fluid flow, oxygen tension etc. in a sterile, closed circulation loop.
Tags
Chip-basedThree-dimensional Cell CulturePerfused Micro-bioreactorsChipNon-biodegradable PolymersBio-degradable PolymersDimensionsCubic Micro-containersRound RecessesScaffoldCellsNutrient SupplyGas SupplyBioreactor HousingClosed Steril Circulation LoopRoller PumpMedium ReservoirPerfusionSuperfusionMixed Operation ModeCell LinesPrimary CellsOrganotypic FunctionsHepatocellular Carcinoma Cell LinesLiver Specific Genes
