/
/
Levande bilder av GLUT4 Protein människohandel mus primära hypotalamus nervceller använder Deconvolution mikroskopi
This content is Free Access.
1The Ph.D. Program for Cancer Biology and Drug Discovery, College of Medical Science and Technology,Taipei Medical University, 2Integrated Laboratory, Center of Translational Medicine,Taipei Medical University, 3Core Facility,Taipei Medical University, 4The Ph.D. Program for Neural Regenerative Medicine, College of Medical Science and Technology,Taipei Medical University, 5Graduate Institute of Neural Regenerative Medicine, College of Medical Science and Technology,Taipei Medical University, 6TMU research center for Neurotrauma and Neuroregeneration, College of Medical Science and Technology,Taipei Medical University
Capitoli
- 00:05Titolo
- 01:00Primary Neuron Culture and Brain Tissue Isolation
- 02:56Tissue Digestion, Plating, and Culture
- 04:32Live Image Recording
- 06:48Results: The Snapshots of GFP-GLUT4 in Hypothalamic Neurons
- 07:47Conclusion
Det här protokollet beskriver en teknik för observation av realtid grönt fluorescerande Protein (GFP) taggade glukos Transporter 4 (GLUT4) protein människohandel på insulin stimulans och karakterisering av CCR5 biologiska roll i det insulin – GLUT4 signalering utbildningsavsnitt med Deconvolution mikroskopi.
