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Measuring the Effect of Chemicals on the Growth and Reproduction of Caenorhabditis elegans
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JoVE Journal Medicina
Measuring the Effect of Chemicals on the Growth and Reproduction of Caenorhabditis elegans

Measuring the Effect of Chemicals on the Growth and Reproduction of Caenorhabditis elegans

DOI:
10.3791/56437-v

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08:12 min

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October 05, 2017

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,
1Systems Biotechnology Research Center,Korea Institute of Science and Technology

Capitoli

  • 00:05Titolo
  • 00:55Preparation of NGM Plates Containing Either 1% DMSO or 750 μM Etoposide
  • 03:44Measurement of C. elegans Growth
  • 05:26C. elegans Egg Laying Assay
  • 06:18Results: C. elegans Growth and Reproduction with Etoposide Treatment
  • 07:41Conclusion

Summary

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A basic protocol to evaluate the toxicity of chemicals in a model animal, Caenorhabditis elegans, is described. The method is convenient and useful for the development of pharmaceuticals as well as for the risk assessment of various environmental pollutants.

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Keywords: Caenorhabditis ElegansChemical EffectsGrowthReproductionToxicologyEtoposideDMSONGM MediumE. Coli OP50Age-synchronized Eggs

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