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Measuring Influenza Neutralizing Antibody Responses to A(H3N2) Viruses in Human Sera by Microneutralization Assays Using MDCK-SIAT1 Cells
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JoVE Journal Immunologia e infezioni
Measuring Influenza Neutralizing Antibody Responses to A(H3N2) Viruses in Human Sera by Microneutralization Assays Using MDCK-SIAT1 Cells

Measuring Influenza Neutralizing Antibody Responses to A(H3N2) Viruses in Human Sera by Microneutralization Assays Using MDCK-SIAT1 Cells

DOI:
10.3791/56448-v

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12:09 min

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November 22, 2017

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, , , ,
1Influenza Division,Centers for Disease Control and Prevention, 2Battelle

Capitoli

  • 00:05Titolo
  • 01:09Determination of Tissue-culture Infectious Dose (TCID) of the Virus
  • 05:44Microneutralization (MN) Assay Using MDCK-SIAT1 Cells
  • 09:44Results: Microneutralization Assay Results of Human Sera tested Against A/HongKong/4801/2014 A(H3N2) Virus Using MDCK-SIAT1 Cells
  • 11:25Conclusion

Summary

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Traduzione automatica

Influenza neutralizing antibodies correlate with protection of influenza infections. Microneutralization assays measure neutralizing antibodies in human sera and are often used for influenza human serology. We describe a microneutralization assay using MDCK-SIAT1 cells to measure neutralizing antibody titers to contemporary 3C.2a and 3C.3a A(H3N2) viruses following influenza vaccination or infection.

Tags

InfluenzaNeutralizing AntibodyMicroneutralization AssayMDCK-SIAT1 CellsH3N2 VirusesVirus PropagationVirus DilutionELISANucleoprotein Monoclonal Antibody

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