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A RANKL-based Osteoclast Culture Assay of Mouse Bone Marrow to Investigate the Role of mTORC1 in Osteoclast Formation
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1Department of Pediatric Dentistry, Ninth People’s Hospital, Shanghai Jiaotong University School of Medicine, Shanghai Key Laboratory of Stomatology & Shanghai Research Institute of Stomatology,National Clinical Research Center of Stomatology, 2State Key Laboratory of Cell Biology, CAS Center for Excellence in Molecular Cell Science, Shanghai Institute of Biochemistry and Cell Biology,University of Chinese Academy of Sciences, 3Department of Oral and Maxillofacial-Head and Neck Oncology, Ninth People’s Hospital,Shanghai Jiaotong University School of Medicine
Capitoli
- 00:05Titolo
- 00:59Dissection and Isolation
- 03:27Plating and Differentiation
- 05:41Tartrate Resistant Acid Phosphatase Staining and Activity Quantification of Culture Medium
- 07:25Results: Deletion of Raptor Impaired Osteoclast Formation
- 08:27Conclusion
