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一种基于阵列的比较基因组杂交平台, 有效检测快中子诱导的苜蓿蒺藜突变体的拷贝数变化
JoVE Journal
Genetica
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JoVE Journal Genetica
An Array-based Comparative Genomic Hybridization Platform for Efficient Detection of Copy Number Variations in Fast Neutron-induced Medicago truncatula Mutants
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一种基于阵列的比较基因组杂交平台, 有效检测快中子诱导的苜蓿蒺藜突变体的拷贝数变化

DOI:
10.3791/56470-v

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09:32 min

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November 08, 2017

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, , , , ,
1Laboratory of Plant Genetics and Development,Noble Research Institute, 2Genetics Laboratory,University of Oklahoma Health Science Center, 3Medicine and Health School,Li Shui University

Capitoli

  • 00:05Titolo
  • 01:00DNA Isolation, Labeling, and Purification
  • 04:04Hybridization, Washing, and Scanning of the Genome Arrays
  • 06:45Results: Array-based Comparative Genome Hybridization to Detect Copy Number Variations in Medicago truncatula Mutants
  • 08:09Conclusion

Summary

Traduzione automatica

Traduzione automatica

该协议提供了实验步骤和有关试剂、设备和分析工具的信息, 这些研究人员有兴趣执行整个基因组阵列的比较基因组杂交 (计算全息) 对拷贝数变化的分析植物.

Tags

Array-based Comparative Genomic HybridizationCopy Number VariationsFast Neutron-induced MutantsMedicago TruncatulaLegume BiologyNodule DevelopmentDNA ExtractionDNA LabelingDNA Purification

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