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Focal Macropatch Recordings of Synaptic Currents from the Drosophila Larval Neuromuscular Junction
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JoVE Journal Neuroscienze
Focal Macropatch Recordings of Synaptic Currents from the Drosophila Larval Neuromuscular Junction

Focal Macropatch Recordings of Synaptic Currents from the Drosophila Larval Neuromuscular Junction

DOI:
10.3791/56493-v

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07:01 min

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September 25, 2017

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1Department of Neurology, School of Medicine,Wayne State University, 2Department of Anatomy and Cell Biology, School of Medicine,Wayne State University

Capitoli

  • 00:05Titolo
  • 00:39Fabrication of Recording Electrodes
  • 01:53Additional Preparation
  • 03:17EJC Electrical Recording
  • 04:33EJC Electrical Stimulation and Seal Resistance
  • 05:34Results: Accurate Detection of Release Events
  • 06:29Conclusion

Summary

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Synaptic currents can be recorded focally from visualized synaptic boutons at the Drosophila third instar larvae neuromuscular junction. This technique enables monitoring the activity of a single synaptic bouton.

Tags

DrosophilaLarval Neuromuscular JunctionSynaptic CurrentsFocal Macropatch RecordingsSynaptic BoutonsSynaptic TransmissionSynaptic ProteinsMicroelectrodeHemolymph-like SolutionHL3 SolutionStimulation PipettesDissection PlatesWandering Third Instar LarvaeFiletingPinningNerve Cutting

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