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Subcellular Fractionation for ERK Activation Upon Mitochondrial-derived Peptide Treatment
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JoVE Journal Biologia
Subcellular Fractionation for ERK Activation Upon Mitochondrial-derived Peptide Treatment

Subcellular Fractionation for ERK Activation Upon Mitochondrial-derived Peptide Treatment

DOI:
10.3791/56496-v

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07:55 min

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September 25, 2017

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, , ,
1Leonard Davis School of Gerontology,University of Southern California

Capitoli

  • 00:05Titolo
  • 00:52Peptide Treatment to Cells
  • 01:46Subcellular Fractionation of Cytosol and Nucleus
  • 03:31Isolate Crude Mitochondrial Fraction
  • 05:43Results: Analysis of ERK Activation Upon Mitochondrial-derived Peptide Treatment
  • 07:17Conclusion

Summary

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This protocol describes how to stimulate cells with mitochondrial-derived peptides and assess the signaling cascade and localization of phospho-proteins.

Tags

Keywords: Subcellular FractionationERK ActivationMitochondrial-derived PeptideSignaling CascadeHumaninSH-SY5Y CellsHEK293 CellsCytoplasmic FractionNuclear FractionCytosol FractionNuclear Extraction Buffer

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