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天然铀对体外破影响的分析方法

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Biologia dello sviluppo

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JoVE Journal Biologia dello sviluppo

Methods for Analyzing the Impacts of Natural Uranium on In Vitro Osteoclastogenesis

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天然铀对体外破影响的分析方法

DOI:

10.3791/56499-v

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09:51 min

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January 30, 2018

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Tatiana Gritsaenko, Valérie Pierrefite-Carle, Gaëlle Creff, Claude Vidaud, Georges Carle, Sabine Santucci-Darmanin

¹UMR E-4320 TIRO-MATOs CEA/DRF/BIAM,Université Nice Sophia-Antipolis, ²UMR 7272 Institut de Chimie de Nice CNRS,Université Nice Sophia-Antipolis, ³CEA, Direction de la Recherche Fondamentale (DRF),Biosciences and Biotechnologies Institute (BIAM)

Capitoli

00:05Titolo
00:38RAW 264.7 Differentiation in the Presence of Uranyl Ions [U(VI)]
03:14Osteoclast Size and Morphology Analyses
05:27Osteoclast Function in the Presence of U(VI)
08:30Results: Representative Effects of U(VI) on Osteoclast Size and Function
09:34Conclusion

Summary

Traduzione automatica

众所周知, 铀会影响骨骼的新陈代谢。在这里, 我们提出了一项协议, 目的是研究天然铀暴露对破骨细胞的活性、分化和功能的影响, 这是负责骨质吸收的单元。

Tags

Osteoclastogenesis Uranium Exposure RAW 264.7 Cells Cell Culture Cell Seeding Density Uranium Salt Solution RANKL Osteoclast Differentiation Osteoclast Function Environmental Toxicity Bone Physiology Bone Metabolism

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