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En automatiseret hurtige iterativ Negative Geotaxis analyse til at analysere voksen klatring adfærd i en Drosophila Model af Neurodegeneration
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1Research Center for Translational Medicine,Tongji University School of Medicine, 2Key Laboratory of Arrhythmias of the Ministry of Education of China, Shanghai East Hospital,Tongji University School of Medicine, 3Department of Anatomy and Neurobiology,Tongji University School of Medicine, 4Shanghai Advanced Research Institute, University of Chinese Academy of Sciences,Chinese Academy of Sciences, 5Sino-Danish College, University of Chinese Academy of Sciences,Chinese Academy of Sciences
Capitoli
- 00:05Titolo
- 01:14Fly Collection
- 02:13Automated RING Assay
- 03:56RING Assay Data Analysis
- 05:19Results: Adult Climbing Behavior in Drosophila melanogaster
- 06:03Conclusion
Denne trinvise protokol analyserer Drosophila negativt geotaxis adfærd ved hjælp af en automatiseret multi-cylindret system, der er vært for hundredvis af fluer og synkroniserer deres handling af en elektromotor. Ved synkronisering, er flyve negativt geotaxis adfærd analyseret, digitalt optaget, og analyseres ved hjælp af selvstændige designet RflyDetection software.
