/

Immunologia e infezioni

/

巨噬细胞中含有吞噬的鼠伤寒沙门氏菌的分离

JoVE Journal
Immunologia e infezioni

È necessario avere un abbonamento a JoVE per visualizzare questo. Accedi o inizia la tua prova gratuita.

JoVE Journal Immunologia e infezioni

Isolation of Salmonella typhimurium-containing Phagosomes from Macrophages

Please note that all translations are automatically generated. Click here for the English version.

巨噬细胞中含有吞噬的鼠伤寒沙门氏菌的分离

DOI:

10.3791/56514-v

•

10:33 min

•

October 25, 2017

•

Saray Gutiérrez², Martina Wolke, Georg Plum, Nirmal Robinson²

¹Institute for Medical Microbiology, Immunology and Hygiene,University of Cologne, ²Cologne Cluster of Excellence in Cellular Stress Responses in Aging-Associated Diseases (CECAD),University of Cologne

Capitoli

00:05Titolo
01:03Salmonella typhimurium Culture
02:17Coating of Bacteria with NHS-biotin/Streptavidin-conjugated Magnetic Beads
04:38Bone Marrow-derived Macrophages (BMDM) Infection
06:18Isolation of S. typhimurium-containing Phagosomes
08:21Results: Successful Biotinylation of Bacterial and Isolation of Bacterial-containing Phagosomes
09:41Conclusion

Summary

Traduzione automatica

我们在这里描述了一个简单和快速的方法, 以隔离的鼠伤寒沙门氏菌-含有吞噬从巨噬细胞涂层细菌与生物素和亲和。

Tags

Salmonella Typhimurium Phagosomes Macrophages Isolation Immunology Antigen Processing Intracellular Pathogens Biotin Streptavidin-conjugated Magnetic Beads

Article

Embed

AGGIUNGI ALLA PLAYLIST

Usage Statistics

Video correlati

从老鼠肠道树突状细胞和巨噬细胞的分离和表征

一个协议感染<em>秀丽隐杆线虫</em>与<em>鼠伤寒沙门氏菌</em

高通量检测到的表型<em>沙门氏菌</em>伤寒协会，侵袭和复制的巨噬细胞

可视化的非溶胞胞吐作用<em>新型隐球菌</em>从巨噬细胞用数码光学显微镜

胞浆的定量与液泡<em>沙门氏菌</em>在原发性巨噬细胞差透

通过流式细胞仪大鼠肾脏巨噬细胞的表型特征

嗜肺军团菌外膜囊泡：隔离及对巨噬细胞促炎潜力分析

受肥胖相关炎症组织的巨噬细胞的分离、鉴定和纯化

小鼠与人肺泡巨噬细胞的分离和体外培养

慢性沙门氏菌感染诱发肠纤维化

Read Article