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应用流式细胞仪分析单侧坐骨神经和背根神经节的免疫细胞

JoVE Journal
Neuroscienze

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JoVE Journal Neuroscienze

Analysis of Immune Cells in Single Sciatic Nerves and Dorsal Root Ganglion from a Single Mouse Using Flow Cytometry

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应用流式细胞仪分析单侧坐骨神经和背根神经节的免疫细胞

DOI:

10.3791/56538-v

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12:34 min

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December 06, 2017

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Asa S. Hidmark, Peter P. Nawroth^2,3, Thomas Fleming³

¹Department of Medicine I and Clinical Chemistry,University Hospital of Heidelberg, ²Institute for Diabetes and Cancer IDC Helmholtz Center Munich,Germany & Joint Heidelberg-IDC Translational Diabetes Program, ³German Center for Diabetes Research (DZD)

Capitoli

00:05Titolo
01:28Dissection of Sciatic Nerve and Dorsal Root Ganglion
04:02Digestion of Sciatic Nerve and Dorsal Root Ganglion
06:36Setup and Flow Cytometry and Running of Samples
10:00Results: Flow Cytometry Analysis of Surface Expressed Markers on Singlets from the Sciatic Nerve and Dorsal Root Ganglion
11:46Conclusion

Summary

Traduzione automatica

由于组织的缺乏, 小鼠坐骨神经内细胞含量的定量分析是困难的。本协议描述了一种组织消化和制备的方法, 它提供了足够的细胞, 用于对单个小鼠神经的免疫细胞进行流式细胞仪分析。

Tags

Keywords: Flow Cytometry Immune Cells Sciatic Nerve Dorsal Root Ganglion Neuropathy Neuroinflammation Mouse Dissection Cell Isolation Digestion

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