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人线粒体 DNA 中核苷酸的同时定位与定量

JoVE Journal
Biologia

This content is Free Access.

JoVE Journal Biologia

Simultaneous Mapping and Quantitation of Ribonucleotides in Human Mitochondrial DNA

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人线粒体 DNA 中核苷酸的同时定位与定量

DOI:

10.3791/56551-v

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12:35 min

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November 14, 2017

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Katrin Kreisel, Martin K.M. Engqvist², Anders R. Clausen

¹Department for Medical Biochemistry and Cell Biology,University of Gothenburg, ²Department of Biology and Biological Engineering,Chalmers University of Technology

Capitoli

00:05Titolo
00:55HincII Treatment and Alkaline Hydrolysis
04:115′-End Phosphorylation
05:21Single Strand DNA Ligation
06:35Second Strand Synthesis
08:23PCR Amplification, Library Analysis and Pooling
10:10Results: Simultaneous Quantitation and Genome-wide Mapping of Ribonucleotides
11:39Conclusion

Summary

Traduzione automatica

在这里, 我们描述一个方法可以同时定量和基因组范围内的地图核苷酸在高度完整的 dna 在核苷酸决议, 结合酶裂解的基因组 dna 与其碱性水解和随后5´端序.

Tags

Keywords: Mitochondrial DNA Ribonucleotides DNA Integrity DNA Replication DNA Repair HeLa Cells HincII Paramagnetic Beads 70% Ethanol Potassium Hydroxide Potassium Chloride Sodium Acetate

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