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主动脉-性腺-肾外植体培养体系在发育性造血中的应用

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Biologia dello sviluppo

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JoVE Journal Biologia dello sviluppo

Application of Aorta-gonad-mesonephros Explant Culture System in Developmental Hematopoiesis

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主动脉-性腺-肾外植体培养体系在发育性造血中的应用

DOI:

10.3791/56557-v

•

07:38 min

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November 03, 2017

•

Junhua Lv², Feng Liu²

¹State Key Laboratory of Membrane Biology, Institute of Zoology,Chinese Academy of Sciences, ²University of Chinese Academy of Sciences

Capitoli

00:05Titolo
01:06Aorta-gonad-mesonephros (AGM) Explant Culture and Colony Forming Units CFU) Analysis |
04:24In Vivo AGM Transplantation
06:08Results: Representative Effects of Fluoxetine on Hematopoietic Stem Cell (HSC) Development
07:08Conclusion

Summary

Traduzione automatica

本议定书描述使用培养的主动脉-性腺-肾的表达分析, 菌落形成单位在文化和脾脏, 并长期重建, 以确定影响的调节因子和信号通路的造血干细胞发育。这已被证明是一个有效的系统研究造血干细胞生物学和功能。

Tags

Keywords: Aorta-gonad-mesonephros Explant Culture Hematopoiesis Hematopoietic Stem Cells HSC Development Embryonic Dissection Collagenase Cell Culture M3434 Medium

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