Rivista
/
Ricerca sul cancro
/
基于细胞外基质提取物 (ECME) 的三维系统中单核细胞与原代乳癌细胞的通讯分析
JoVE Journal
Ricerca sul cancro
È necessario avere un abbonamento a JoVE per visualizzare questo.  Accedi o inizia la tua prova gratuita.
JoVE Journal Ricerca sul cancro
Analyzing the Communication Between Monocytes and Primary Breast Cancer Cells in an Extracellular Matrix Extract (ECME)-based Three-dimensional System
Please note that all translations are automatically generated. Click here for the English version.

基于细胞外基质提取物 (ECME) 的三维系统中单核细胞与原代乳癌细胞的通讯分析

DOI:
10.3791/56589-v

•

10:55 min

•

January 08, 2018

•

, ,
1Unidad de Investigación en Virología y Cáncer,Hospital Infantil de México Federico Gómez, 2Programa de Doctorado en Ciencias Biomédicas, Facultad de Medicina,Universidad Nacional Autónoma de México

Capitoli

  • 00:05Titolo
  • 01:01Establishing 3D Co-cultures with Indirect Interaction
  • 02:133D Co-cultures with Direct Interaction
  • 06:14Characterization of the Effects of Supernatants from Primary BrC Cells on Acini Formation and Acini Structure
  • 08:28Results: Communication between Monocytes and Primary BrC Cells in an ECME-3D System
  • 10:01Conclusion

Summary

Traduzione automatica

Traduzione automatica

在这里, 我们描述了一个三维的培养方法来分析原发性乳腺癌细胞的形态学, 以及研究他们的直接/间接相互作用的单核和结果, 如胶原降解, 免疫细胞招募, 细胞侵袭和促进癌症相关的炎症。

Tags

3D Co-cultureMonocyte-breast Cancer Cell CommunicationTumor MicroenvironmentExtracellular Matrix Extract (ECME)U937 CellsTHP-1 CellsPrimary Breast Cancer CellsCell LabelingFluorescent DyesTrypsinization

Article

Embed

AGGIUNGI ALLA PLAYLIST

Usage Statistics

Video correlati

check_url/it/56589?article_type=v

利用功能基因组筛选识别在肿瘤细胞球体培养潜在的新药物靶标
check_url/it/56589?article_type=v

向上移动: 一个简单而灵活的体外三维入侵检测协议
check_url/it/56589?article_type=v

骨肉瘤前小鼠模型对肿瘤与间充质干细胞细胞外囊泡介导通信的定义
check_url/it/56589?article_type=v

舌细胞外基质的制备与舌鳞癌体外重建
check_url/it/56589?article_type=v

生成毛因诱导多能干细胞衍生的肿瘤抗原特异性胸腺移民的三维胸腺培养系统
check_url/it/56589?article_type=v

骨肉瘤的三维骨细胞外基质模型
check_url/it/56589?article_type=v

利用成像质谱技术捕获组织与细胞之间的小分子通信
check_url/it/56589?article_type=v

三维流状入侵检测中的全人肿瘤基基质
check_url/it/56589?article_type=v

小鼠乳腺皮下HC11和EpH4细胞的分化
check_url/it/56589?article_type=v

使用微生理系统模拟人体乳房组织中的乳腺癌

Read Article