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Multiplexed Immunofluorescence Analysis and Quantification of Intratumoral PD-1+ Tim-3+ CD8+ T Cells
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JoVE Journal Ricerca sul cancro
Multiplexed Immunofluorescence Analysis and Quantification of Intratumoral PD-1+ Tim-3+ CD8+ T Cells

Multiplexed Immunofluorescence Analysis and Quantification of Intratumoral PD-1+ Tim-3+ CD8+ T Cells

DOI:
10.3791/56606-v

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09:32 min

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February 08, 2018

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, , , , , , , , , , , , , , , ,
1PARCC-INSERM U970,Université Paris Descartes, 2INSERM U970,Université Paris Descartes, 3Equipe Labellisée Ligue Contre le Cancer, 4Department of Immunology,Hopital Européen Georges Pompidou, 5Department of Medical Urology,Hopital Européen Georges Pompidou, 6Department of Pathology,Hopital Européen Georges Pompidou, 7Université Paris Diderot Paris 7, 8Department of medical oncology,Hopital Européen Georges Pompidou

Capitoli

  • 00:04Titolo
  • 00:59Immunostaining
  • 02:56Slide Scanning
  • 04:30Image Analysis
  • 07:16Results: Representative Intratumoral PD-1+Tim-3+CD8+ T cell Quantification and Analysis
  • 08:51Conclusion

Summary

Traduzione automatica

Traduzione automatica

Studying tumor microenvironment may identify prognostic or predictive biomarkers of clinical response to immunotherapy. Presented here, is an innovative method based on in situ fluorescence multispectral imaging to analyze and count automatically various subpopulations of CD8+ T cells. This reproducible and reliable technique is suitable for large cohort analyses.

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Keywords: Multiplexed ImmunofluorescenceCell CountingTumor MicroenvironmentPhenotypingPD-1Tim-3CD8+ T CellsImmunological InteractionsAntibody LabelingImage Scanning

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