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血管动力尾静脉注射对小鼠肝细胞基因本构和诱导系统的修饰
JoVE Journal
Genetica
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JoVE Journal Genetica
Constitutive and Inducible Systems for Genetic In Vivo Modification of Mouse Hepatocytes Using Hydrodynamic Tail Vein Injection
DOI:

09:35 min

February 02, 2018

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Capitoli

  • 00:05Titolo
  • 00:52Recombinatorial Cloning to Generate Ready-for-injection Clones
  • 02:23Hydrodynamic Tail Vein Injection
  • 04:22Induction of Transfected CreER with Tamoxifen
  • 05:33Induction of Tetracycline-dependent Gene or shRNA Expression
  • 06:35Preparation of Mouse Liver for Analysis by Immunostaining
  • 07:30Results: Constitutive and Inducible Gene Expression in the Liver Following HTVI
  • 08:47Conclusion

Summary

Traduzione automatica

水动力尾静脉注射转的融合载体, 可以稳定转染小鼠肝细胞在体内.在这里, 我们提出了一个实用的转染系统的协议, 使一个单一的转基因或联合本构和强力霉素诱导表达的转基因或和平 shRNA 在肝脏的长期本构表达。

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