/
/
Forbedret 3D Hydrogel kulturer av primære gliacellene for In Vitro modellering av Neuroinflammation
È necessario avere un abbonamento a JoVE per visualizzare questo. Accedi o inizia la tua prova gratuita.
1Department of Psychiatry,University of Alberta, 2Alberta Innovates-Health Solutions Interdisciplinary Team in Smart Neural Prostheses (Project SMART),University of Alberta, 3Department of Chemical and Materials Engineering,University of Alberta, 4Division of Physical Medicine and Rehabilitation,University of Alberta, 5Centre for Neuroscience,University of Alberta
Capitoli
- 00:05Titolo
- 00:43Coverslip and Mold Preparation
- 02:27Preparation of Cells for Encapsulation
- 04:38Cell Encapsulation
- 06:58Results: Confocal Microscopy and 2D and 3D Mixed Cultures
- 08:47Conclusion
Her presenterer vi en protokoll for 3D kultur rotte hjerne-avledet glia cellene, inkludert astrocyttene, microglia og oligodendrocytes. Viser vi primære cellekultur, methacrylated hyaluronsyre (HAMA) hydrogel syntese, HAMAphoto-polymerisasjon og celle innkapsling og eksempel behandling for AC confocal og skanning elektron mikroskopiske bildebehandling.
