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点化学法现场定向固定骨形态发生蛋白2到固体表面
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Bioingegneria
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JoVE Journal Bioingegneria
Site-Directed Immobilization of Bone Morphogenetic Protein 2 to Solid Surfaces by Click Chemistry
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点化学法现场定向固定骨形态发生蛋白2到固体表面

DOI:
10.3791/56616-v

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11:20 min

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March 29, 2018

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, , , , , ,
1Fraunhofer-Institut für Grenzflächen- und Bioverfahrenstechnik (IGB), Translationszentrum Würzburg ‘Regenerative Therapien für Krebs- und Muskuloskelettale Erkrankung’,Institutsteil Würzburg, 2Lehrstuhl für Tissue Engineering und Regenerative Medizin,Universitätsklinikum Würzburg, 3Lehrstuhl für Pharmazeutische Technologie und Biopharmazie,Universität Würzburg, 4Lehrstuhl für molekulare Pflanzenphysiologie und Biophysik, Julius-von-Sachs Institut für Biowissenschaften,Universität Würzburg

Capitoli

  • 00:04Titolo
  • 00:46Expression and Purification of BMP2-K3Plk
  • 05:46Covalent Coupling Technique of BMP2-K3Plk to Azide Functionalized Agarose Beads
  • 07:04Measuring Alkaline Phosphatase (ALP) Expression to Prove the In Vitro Bioactivity of the Produced BMP2-K3Plk Before and After the Coupling Reaction
  • 08:34Results: Evaluation of the Bioactivity of BMP2-K3Plk after Site-Directed Immobilization
  • 09:54Conclusion

Summary

Traduzione automatica

Traduzione automatica

用骨形态发生蛋白 2 (BMP2) 掺杂的生物材料作为治疗非愈合性骨折的新方法。为了克服因无法控制的释放因素而产生的副作用, 我们提出了一个新的策略, 以现场直接固定的因素, 从而创造材料, 改善成骨能力。

Tags

Keywords: Site-directedImmobilizationBone Morphogenetic Protein 2BMP2Click ChemistryOsteogenic ScaffoldNon-healing Long Bone DefectsCovalent CouplingAbsorptionEncapsulationRecombinant Protein ExpressionBacterial CultureProtein PurificationSonicationCentrifugation

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