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吸收荧光、磁性纳米微粒测定昆虫的吸液能力

JoVE Journal
Biologia

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JoVE Journal Biologia

The Ingestion of Fluorescent, Magnetic Nanoparticles for Determining Fluid-uptake Abilities in Insects

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吸收荧光、磁性纳米微粒测定昆虫的吸液能力

DOI:

10.3791/56619-v

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07:03 min

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December 20, 2017

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Matthew S. Lehnert, Kristen E. Reiter, Andrew Bennett, Patrick D. Gerard, Qi-Huo Wei, Miranda Byler, Huan Yan, Wah-Keat Lee

¹Department of Biological Sciences,Kent State University at Stark, ²Department of Mathematical Sciences,Clemson University, ³Liquid Crystal Institute,Kent State University, ⁴Brookhaven National Laboratory

Capitoli

00:05Titolo
00:38Insect Species, Preparation of Solutions, and Feeding Station Setup
01:43Feeding Protocol
03:05Dissections
04:31Testing for Ingested Nanoparticles
05:24Results: A Relationship Between Feeding Pore Size and Feeding Conduit Size
06:28Conclusion

Summary

Traduzione automatica

液体喂养的昆虫有能力从多孔表面获取少量液体。该协议描述了一种方法, 直接确定昆虫的能力, 从多孔表面摄取液体使用荧光, 磁性纳米微粒的喂养解决方案。

Tags

Fluorescent Magnetic Nanoparticles Fluid-feeding Insects Insect Mouth Parts Insect Feeding Mechanisms Blue Bottle Flies House Flies Cabbage Butterflies Sucrose Solution Feeding Station Nylon Net Filters Membrane Filters Microscope Slides Manipulator Feeding Stage Filter Paper Insect Pin

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