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In Vivo EPR Assessment of pH, pO2, Redox Status, and Concentrations of Phosphate and Glutathione in the Tumor Microenvironment
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In Vivo EPR Assessment of pH, pO2, Redox Status, and Concentrations of Phosphate and Glutathione in the Tumor Microenvironment

In Vivo EPR Assessment of pH, pO2, Redox Status, and Concentrations of Phosphate and Glutathione in the Tumor Microenvironment

DOI:
10.3791/56624-v

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10:46 min

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March 16, 2018

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, , ,
1In Vivo Multifunctional Magnetic Resonance center, Robert C. Byrd Health Sciences Center,West Virginia University, 2Department of Biochemistry,West Virginia University School of Medicine, 3Department of Microbiology, Immunology & Cell Biology,West Virginia University School of Medicine

Capitoli

  • 00:04Titolo
  • 01:20Probe Synthesis and Calibration
  • 05:42Probe Delivery for In Vivo Functional Measurements
  • 07:18In Vivo Functional Measurements
  • 08:09Results: EPR Assessment in the Tumor Microenvironment
  • 10:04Conclusion

Summary

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Low-field (L-band, 1.2 GHz) electron paramagnetic resonance using soluble nitroxyl and trityl probes is demonstrated for assessment of physiologically important parameters in the tumor microenvironment in mouse models of breast cancer.

Tags

In Vivo EPRTumor MicroenvironmentPhysiological ParametersOxygen TensionRedox StatusPhosphateGlutathioneNoninvasive AssessmentParamagnetic ProbesOxygen-sensitive ProbePH And Redox ProbeCalibrationEPR Spectroscopy

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