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Capitoli
- 00:00Titolo
- 02:47Preparation of Pipettes of ISE
- 04:02Preparation of Patch-clamp Pipette
- 05:40Preparation of Cells
- 05:59Recording of Transporter Activity
- 10:01Representative Results
- 11:19Conclusion
This manuscript describes the applications of proton-selective electrodes and patch clamping methods to measure the activity of proton transport systems. These methods overcome some limitations of techniques commonly used to study proton transport activity, such as moderate sensitivity, time resolution and insufficient intracellular milieu control.
