Rivista
/
Medicina
/
Isolation and Cultivation of Adult Rat Cardiomyocytes
JoVE Journal
Medicina
È necessario avere un abbonamento a JoVE per visualizzare questo.  Accedi o inizia la tua prova gratuita.
JoVE Journal Medicina
Isolation and Cultivation of Adult Rat Cardiomyocytes

Isolation and Cultivation of Adult Rat Cardiomyocytes

DOI:
10.3791/56634-v

•

11:06 min

•

October 19, 2017

•

, ,
1Institute of Physiology,Justus Liebig University Giessen

Capitoli

  • 00:05Titolo
  • 01:15Preparation of Langendorff Perfusion System
  • 03:09Extraction and Isolation of the Heart
  • 08:21Results: The Appearance and Behavior of Adult Rat Ventricular Cardiomyocytes (ARVC) during Six Days of Cultivation
  • 10:02Conclusion

Summary

Traduzione automatica

Traduzione automatica

Here, we present a protocol for the isolation and cultivation of adult rat ventricular cardiomyocytes (ARVC). Isolated ARVC can be used for short and long-term cultivation. The isolation and cultivation of ARVC can play a key role in developing new treatment regimens for cardiac diseases.

Tags

Adult Rat CardiomyocytesCardiac BiologyCardiac PathophysiologyCardiac DedifferentiationCardiac HypertensionHeart FailureLangendorff Perfusion SystemCollagenasePowell MediumWistar Rats

Article

Embed

AGGIUNGI ALLA PLAYLIST

Usage Statistics

Video correlati

Isolation and Functional Characterization of Human Ventricular Cardiomyocytes from Fresh Surgical Samples

Isolation of Atrial Cardiomyocytes from a Rat Model of Metabolic Syndrome-related Heart Failure with Preserved Ejection Fraction

An Efficient Sieving Method to Isolate Intact Glomeruli from Adult Rat Kidney

Isolation of Atrial Myocytes from Adult Mice

Murine Myocardial Infarction Model using Permanent Ligation of Left Anterior Descending Coronary Artery

Ultrasonic-augmented Primary Adult Fibroblast Isolation

In Vitro Assessment of Cardiac Function Using Skinned Cardiomyocytes

Isolation and Cultivation of Mandibular Bone Marrow Mesenchymal Stem Cells in Rats

Sarcomere Shortening of Pluripotent Stem Cell-Derived Cardiomyocytes using Fluorescent-Tagged Sarcomere Proteins.

Modifications of the Langendorff Method for Simultaneous Isolation of Atrial and Ventricular Myocytes from Adult Mice

Read Article