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大鼠视网膜仿生化学调节的神经递质谷氨酸的体外实验方法
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Bioingegneria
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JoVE Journal Bioingegneria
Methodology for Biomimetic Chemical Neuromodulation of Rat Retinas with the Neurotransmitter Glutamate In Vitro
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大鼠视网膜仿生化学调节的神经递质谷氨酸的体外实验方法

DOI:
10.3791/56645-v

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12:56 min

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December 19, 2017

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, ,
1Department of Mechanical and Industrial Engineering,University of Illinois at Chicago, 2Department of Biomedical Engineering,Northwestern University

Capitoli

  • 00:05Titolo
  • 01:16Wholemount Retinal Preparation
  • 03:12MEA and Data Acquisition Setup
  • 05:23Glutamate Stimulation Preparation
  • 08:38Retinal Recording and Stimulus Program
  • 10:30Results: Electrode Recordings of Neuronal Activity During Visual, Spontaneous, and Glutamate Injection Recordings
  • 12:06Conclusion

Summary

Traduzione automatica

Traduzione automatica

本议定书描述了一种新的方法, 研究一种形式的化学神经的 wholemount 大鼠视网膜体外与神经递质谷氨酸。化学神经是一种有前途的替代传统的电神经视网膜神经元治疗不可逆转性失明引起的感光变性疾病。

Tags

BiomimeticChemical NeuromodulationRat RetinasNeurotransmitterGlutamateIn VitroPhotoreceptor DegenerationRetinal NeuronsArtificial VisionEnucleated EyesAmes’ MediumCorneaLensVitreous HumourScleraRetinaOptic NerveNylon MeshMultielectrode ArrayPMEA SurfacePerfusion

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