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免疫荧光标记的微管和中心蛋白在体内肠道组织和 3D体外肠道 Organoids
JoVE Journal
Biologia dello sviluppo
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JoVE Journal Biologia dello sviluppo
Immuno-fluorescent Labeling of Microtubules and Centrosomal Proteins in Ex Vivo Intestinal Tissue and 3D In Vitro Intestinal Organoids
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免疫荧光标记的微管和中心蛋白在体内肠道组织和 3D体外肠道 Organoids

DOI:
10.3791/56662-v

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09:51 min

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December 13, 2017

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, , , ,
1School of Biological Sciences,University of East Anglia, 2Norwich Medical School,University of East Anglia

Capitoli

  • 00:05Titolo
  • 01:41Isolation of Intestinal Organoids from Basement Matrix Domes in 24-well Plates
  • 03:20Fixation of Isolated Organoids
  • 04:22Blocking, and Primary and Secondary Antibody Incubation
  • 06:41Mounting Isolated Organoids
  • 07:39Results: Isolation and Immunolabeling of Intestinal Organoids
  • 08:55Conclusion

Summary

Traduzione automatica

Traduzione automatica

我们提出了从体内组织和体外基底基质 organoids 分离肠道3D 结构的协议, 并详细介绍了针对微管免疫标记优化的不同固定和染色方案,中心, 和连接蛋白以及细胞标记包括干细胞蛋白 Lgr5。

Tags

Keywords: 3D Intestinal OrganoidsImmunofluorescent LabelingMicrotubulesCentrosomal ProteinsFixationIsolationImmunostaining

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