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通过鱼眼研究糖尿病: 成人 tg (fli: EGFP) 斑马鱼视网膜血管的显微解剖、可视化和分析

JoVE Journal
Medicina

This content is Free Access.

JoVE Journal Medicina

Studying Diabetes Through the Eyes of a Fish: Microdissection, Visualization, and Analysis of the Adult tg(fli:EGFP) Zebrafish Retinal Vasculature

Please note that all translations are automatically generated. Click here for the English version.

通过鱼眼研究糖尿病: 成人 tg (fli: EGFP) 斑马鱼视网膜血管的显微解剖、可视化和分析

DOI:

10.3791/56674-v

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10:07 min

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December 26, 2017

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Lucas Moritz Wiggenhauser, Katharina Kohl, Nadine Dietrich, Hans-Peter Hammes, Jens Kroll

¹Department of Vascular Biology and Tumorangiogenesis, Center for Biomedicine and Medical Technology Mannheim (CBTM), Medical Faculty Mannheim,Heidelberg University, ²V. Medical Clinic, Medical Faculty Mannheim,Heidelberg University

Capitoli

00:05Titolo
00:54Fixation of Zebrafish Tissue
01:41Preparation of the Zebrafish Retinal Vasculature
06:39Mounting and Visualization of the Retinal Vasculature
07:51Results: Analysis of the Adult tg(fli:EGFP) Zebrafish Retinal Vasculature
09:30Conclusion

Summary

Traduzione automatica

在这里, 我们讨论的方法协议, 将允许简单的分析成人 tg (fli: EGFP) 的斑马鱼视网膜血管作为一个快速出的设置长期血管病理与血管和结构的变化。

Tags

Keywords: Tg(fli:EGFP) Zebrafish Retinal Vasculature Microdissection Visualization Analysis Diabetic Retinopathy Microvascular Complications Fluorescence Microscopy Confocal Microscopy PFA Fixation Agarose Embedding Eye Dissection Corneal Puncture Lens Extraction

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