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En simpel cellebaserede immunofluorescens Assay at opdage autoantistof mod N-Methyl-D-aspartat (NMDA) Receptor i blod
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JoVE Journal Neuroscienze
A Simple Cell-based Immunofluorescence Assay to Detect Autoantibody Against the N-Methyl-D-Aspartate (NMDA) Receptor in Blood

En simpel cellebaserede immunofluorescens Assay at opdage autoantistof mod N-Methyl-D-aspartat (NMDA) Receptor i blod

DOI:
10.3791/56676-v

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07:20 min

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January 09, 2018

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,
1Department of Psychiatry,Chang Gung Memorial Hospital-Linkou, 2Department and Graduate Institute of Biomedical Sciences,Chang Gung University

Capitoli

  • 00:05Titolo
  • 00:37Transfection of HEK293 Cells with NR1-GFP Expression Plasmid
  • 02:38Cell-based Immunofluorescence Assay
  • 04:41Results: Anti-NMDA Receptor Autoantibodies Detected in the Blood of Patients with Autoimmune Encephalitis
  • 06:12Conclusion

Summary

Traduzione automatica

Traduzione automatica

Vi udtrykte ectopically NR1 underenhed af NMDA-receptor markeret med grønt fluorescerende protein i humane embryonale celler (HEK293) som antigen til at opdage autoantistoffer mod NMDA receptor i blodet hos patienter mistænkt med autoimmun encephalitis. Denne enkle metode kan være egnet til screening i klinisk indstillinger.

Tags

Cell-based Immunofluorescence AssayNMDA ReceptorAutoantibodyAutoimmune EncephalitisHEK293 CellsNR1-GFP PlasmidTransfectionFluorescence MicroscopyParaformaldehydePBSTSkim Milk

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