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Auditory Brainstem Response und äußeren Haarzellen Whole-Cell Patch Clamp Aufnahme bei postnatalen Ratten
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1Department of Physiology, School of Basic Medical Sciences,Southern Medical University, 2School of Basic Medical Sciences,Guizhou Medical University, 3Department of Laboratory Medicines, Guangzhou General Hospital of Guangzhou Military Region,Southern Medical University, 4Experiment Teaching Center, School of Basic Medical Sciences,Southern Medical University
Capitoli
- 00:04Titolo
- 00:56Auditory Brainstem Response Recording
- 02:12The Organ of Corti Dissection
- 03:22Immunofluorescence Staining
- 05:21Patch Clamp Recording of Isolated OHCs
- 07:33Results: Whole-Cell Patch Clamp Recording of Isolated Outer Hair Cells
- 08:19Conclusion
Dieses Protokoll beschreibt Methoden, um die auditory Brainstem Antwort vom postnatalen Ratte Welpen aufnehmen. Um die funktionelle Entwicklung des äußeren Haarzellen zu untersuchen, ist das experimentelle Verfahren der gesamten Zelle Patch Clamp Aufnahme in isolierten äußeren Haarzellen Schritt für Schritt beschrieben.
