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建立高通量筛选平台评估乳腺癌 HER2 基因扩增的异质性
JoVE Journal
Immunologia e infezioni
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JoVE Journal Immunologia e infezioni
Building Up a High-throughput Screening Platform to Assess the Heterogeneity of HER2 Gene Amplification in Breast Cancers
DOI:

11:34 min

December 05, 2017

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Capitoli

  • 00:05Titolo
  • 01:24Design and Construct Tissue Microarrays (TMAs) Based on the Kononen Technique
  • 06:20Silver In Situ Hybridization (SISH) Analysis of Human Epidermal Growth Factor Receptor 2 (HER2)
  • 09:11Results: Analysis of Heterogeneity of HER2 Gene Amplification in Breast Cancers
  • 10:00Conclusion

Summary

Traduzione automatica

HER2-positive 细胞的异质分布可以在乳腺癌的一个子集中观察到, 并产生临床难题。在这里, 我们介绍了一个可靠的和成本-有效的协议, 以定义, 量化, 并比较 HER2 内肿瘤遗传异质性的大系列异处理乳腺癌。

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