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固定化的线虫线虫分析神经元内细胞的转运
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Biologia dello sviluppo
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JoVE Journal Biologia dello sviluppo
Immobilization of Caenorhabditis elegans to Analyze Intracellular Transport in Neurons
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固定化的线虫线虫分析神经元内细胞的转运

DOI:
10.3791/56690-v

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07:35 min

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October 18, 2017

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1Frontier Research Institute for Interdisciplinary Sciences and Graduate School of Life Sciences,Tohoku University

Capitoli

  • 00:05Titolo
  • 00:34Experimental Preparation
  • 02:00Mounting of Worms
  • 02:56Imaging
  • 05:23Results: Visualization of Axonal and Intraflagellar Transport in Neurons
  • 06:53Conclusion

Summary

Traduzione automatica

Traduzione automatica

线虫线虫(线虫) 是研究轴突和细胞内转运的良好模型。在这里, 我描述了一个协议的在体内记录和分析轴突和 intraflagellar 运输在C. 线虫。

Tags

Keywords: Caenorhabditis ElegansIntracellular TransportNeuronsAxonal TransportIntraflagellar TransportKinesinDyneinMicrotubulesGreen Fluorescent ProteinIn VivoLive ImagingAgarose Gel

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