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昆虫细胞 (sf9) 外源基因在蛋白质功能检测中的瞬态表达
JoVE Journal
Genetica
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JoVE Journal Genetica
Transient Expression of Foreign Genes in Insect Cells (sf9) for Protein Functional Assay
DOI:

11:12 min

February 22, 2018

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Capitoli

  • 00:04Titolo
  • 00:52Insect Cell Culture
  • 01:53Transient Protein Expression
  • 05:00Anti-apoptotic Activity Assay
  • 07:04Cell Viability Assay
  • 08:11Results: Transient Protein Expression Enables Analysis of Anti-apoptotic Activity
  • 09:41Conclusion

Summary

Traduzione automatica

本协议描述了一种热休克诱导蛋白表达系统 (pDHsp/V5-His/sf9 细胞系统), 可用于表达外来蛋白或评价潜在外来蛋白的抗凋亡活性及其截断的氨基酸昆虫细胞中的酸。

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