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从前期到中期二期小鼠卵母细胞进展的染色质传播制剂分析
JoVE Journal
Genetica
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JoVE Journal Genetica
Chromatin Spread Preparations for the Analysis of Mouse Oocyte Progression from Prophase to Metaphase II
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从前期到中期二期小鼠卵母细胞进展的染色质传播制剂分析

DOI:
10.3791/56736-v

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10:39 min

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February 26, 2018

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, ,
1Department of Biochemistry and Molecular Biology,Johns Hopkins University Bloomberg School of Public Health

Capitoli

  • 00:04Titolo
  • 00:59Harvesting Fetal or Neonatal Ovaries
  • 03:55Metaphase I (MI) or Metaphase II (MII) Oocyte Collection
  • 05:17Oocyte Denuding and Zona Pellucida Removal
  • 07:05MI and MII Oocyte Chromatin Spreads
  • 08:08Results: Crossover Formation in C57B/6J Oocytes
  • 09:52Conclusion

Summary

Traduzione automatica

Traduzione automatica

哺乳动物中的卵子发生过程已知易出错, 特别是由于染色体 missegregation。本手稿描述了小鼠前期、中期 I 和 II. 期卵母细胞的染色质传播制备方法。这些基本技术允许研究整个哺乳动物卵子发生过程的染色质束缚蛋白和染色体形态学。

Tags

Chromatin SpreadMouse OocyteProphaseMetaphase IIOogenesisChromosomeImmunolabelingPloidyAneuploidyInfertilityOvaryHypo-extraction BufferSucrose

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