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Visualisere intracellulære SNARE handel af fluorescens levetid Imaging mikroskopi
JoVE Journal
Immunologia e infezioni
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JoVE Journal Immunologia e infezioni
Visualizing Intracellular SNARE Trafficking by Fluorescence Lifetime Imaging Microscopy

Visualisere intracellulære SNARE handel af fluorescens levetid Imaging mikroskopi

DOI:
10.3791/56745-v

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08:55 min

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December 29, 2017

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, , ,
1Department of Tumor Immunology,Radboud University Medical Center

Capitoli

  • 00:05Titolo
  • 00:37Preparation of the Microscopy Samples
  • 01:49Recording of the FLIM Data
  • 03:34Conversion of the Photon Recordings to FLIM Images
  • 04:19Fitting of Fluorescence Lifetime Histograms for Whole-cell FLIM Analysis
  • 06:03Results: Syntaxin 4 and VAMP3 Interaction in Live Cell Visualized by FRET-FLIM
  • 07:43Conclusion

Summary

Traduzione automatica

Traduzione automatica

Denne protokol beskriver en ny metode giver mulighed for kvantitative visualisering af komplekse dannelsen af SNARE proteiner, baseret på Förster resonans energioverførsel og fluorescens levetid imaging mikroskopi.

Tags

Keywords: SNAREFLIMMembrane TraffickingHeLa CellsSyntaxin-4VAMP3Syntaxin-3MCitrineMCherryFluorescence Lifetime Imaging MicroscopyConfocal MicroscopyInstrument Response Function

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