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Establishment of a Primary Culture of Patient-derived Soft Tissue Sarcoma
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JoVE Journal Ricerca sul cancro
Establishment of a Primary Culture of Patient-derived Soft Tissue Sarcoma

Establishment of a Primary Culture of Patient-derived Soft Tissue Sarcoma

DOI:
10.3791/56767-v

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07:55 min

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April 11, 2018

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, , , , , , , , , , ,
1Osteoncology and Rare Tumors Center,Istituto Scientifico Romagnolo per lo Studio e la Cura dei Tumori (IRST) IRCCS, 2Pathology Unit,Morgagni-Pierantoni Hospital, 3Unit of Surgery and Advanced Oncologic Therapies,Morgagni-Pierantoni Hospital

Capitoli

  • 00:04Titolo
  • 01:23Tumor Specimen Collection and Processing
  • 04:03Cytological Sample Preparation
  • 04:41STS Cell Culture
  • 05:42Results: Patient-derived Primary Culture of Liposarcoma Cells
  • 06:41Conclusion

Summary

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The following protocol focuses on the establishment of a primary culture of patient-derived soft tissue sarcoma (STS). This model could help us to better understand the molecular background and pharmacological profile of these rare malignancies and could represent a starting point for further research aimed at improving STS management.

Tags

Keywords: Primary CulturePatient-derivedPreclinical ModelPathophysiologyTherapyDrug TestingCell IsolationTumor HeterogeneityCollagenase DigestionTRIzol Preservation

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