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Dissection and Staining of Drosophila Pupal Ovaries
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JoVE Journal Biologia dello sviluppo
Dissection and Staining of Drosophila Pupal Ovaries

Dissection and Staining of Drosophila Pupal Ovaries

DOI:
10.3791/56779-v

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07:35 min

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March 02, 2018

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, ,
1Department of Biological Sciences,Columbia University, 2Department of Cell and Systems Biology,University of Toronto

Capitoli

  • 00:05Titolo
  • 00:41Egg Laying and Female Larvae Selection
  • 02:12Pupae Antibody Staining Preparation
  • 04:24Immunohistochemistry
  • 05:52Dissecting and Mounting Pupal Ovaries
  • 06:49Results: Clear Antibody Staining that Reveals Drosophila Structure and Cellular Organization
  • 07:12Conclusion

Summary

Traduzione automatica

Traduzione automatica

The Drosophila ovary is an excellent model system for studying stem cell niche development. Though methods for dissecting larval and adult ovaries have been published, pupal ovary dissections require different techniques that have not been published in detail. Here we outline a protocol for dissecting, staining, and mounting pupal ovaries.

Tags

Keywords: DrosophilaPupal OvariesDissectionStainingStem Cell DifferentiationTime Course ExperimentsStem Cell DivisionWandering Third Instar LarvaePhosphate Buffered Saline (PBS)Male And Female LarvaePupariationPre-pupaGlass Pipette TipProtein Glue

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