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以显微镜为基础的方法评估上皮细胞迁移过程中的体外创面愈合

JoVE Journal
Medicina

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JoVE Journal Medicina

Microscopy Based Methods for the Assessment of Epithelial Cell Migration During In Vitro Wound Healing

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以显微镜为基础的方法评估上皮细胞迁移过程中的体外创面愈合

DOI:

10.3791/56799-v

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08:34 min

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January 02, 2018

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Sergio Liarte*¹, Ángel Bernabé-García*¹, David Armero-Barranco, Francisco José Nicolás

¹Laboratorio de Oncología Molecular y TGF-β,IMIB-Arrixaca, ²Departamento de Enfermería, Facultad Enfermería,Universidad de Murcia

Capitoli

00:05Titolo
01:07Artificial Wound Scratch Assay
03:19Artificial Wound Image Analysis and Migration Quantification
04:48Artificial Migration Front Assay
07:07Results: Representative Artificial Wound Scratch and Migration Front Assay Imaging
08:00Conclusion

Summary

Traduzione automatica

本手稿描述了如何在培养的上皮细胞单分子膜切口样病变, 方便模型伤口愈合在体外, 允许成像的共焦或激光扫描显微镜, 并能提供高质量定量和定性数据, 用于研究细胞行为和参与迁移的机制。

Tags

Epithelial Cell Migration In Vitro Wound Healing Artificial Wounding Cell Migration Wound Healing Molecular Markers Artificial Wound Scratch Assay Epithelial Cells Confluency Serum-free Medium Micro-pipette Wound Gap Phase Contrast Microscope CCD Camera Wound Treatments

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