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Skapa en strukturellt realistiska Finita Element geometriska modell av en hjärtmuskelcellen att studera rollen för cellulära arkitekturen i hjärtmuskelcellen systembiologi
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1Cell Structure and Mechanobiology Group,University of Melbourne, 2Systems Biology Laboratory, Melbourne School of Engineering,University of Melbourne, 3Department of Biomedical Engineering,University of Melbourne, 4School of Mathematics and Statistics, Faculty of Science,University of Melbourne, 5Department of Engineering Science,University of Auckland, 6Advanced Microscopy Facility, Bio21 Molecular Science and Biotechnology Institute,University of Melbourne, 7ARC Centre of Excellence in Convergent Bio-Nano Science and Technology,University of Melbourne, 8School of Medicine, Faculty of Medicine, Dentistry and Health Sciences,University of Melbourne, 9Living Systems Institute,University of Exeter
Capitoli
- 00:04Titolo
- 01:19Segment Myofibrils and Mitochondria Regions from the EM 3D Image Dataset
- 03:05Create a Finite Element Mesh from the Segmented Components
- 04:36Mathematically Map the Spatially Varying Density of Ion-channels of Interest onto the Finite Element Mesh
- 06:51Results: A Structurally Realistic Finite Element Geometric Model of a Cardiomyocyte
- 08:13Conclusion
Detta protokoll skisserar en ny metod för att skapa en rumsligt detaljerad finita element modell av intracellulära arkitekturen av hjärtmuskelceller från elektronmikroskopi och konfokalmikroskopi bilder. Kraften i denna rumsligt detaljerad modell demonstreras med hjälp av fallstudier i Kalciumsignalering och blockeringar.
