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Genome-wide Quantification of Translation in Budding Yeast by Ribosome Profiling
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Biochimica
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JoVE Journal Biochimica
Genome-wide Quantification of Translation in Budding Yeast by Ribosome Profiling

Genome-wide Quantification of Translation in Budding Yeast by Ribosome Profiling

DOI:
10.3791/56820-v

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12:57 min

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December 21, 2017

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, , ,
1Department of Dermatology,Boston University School of Medicine

Capitoli

  • 00:05Titolo
  • 00:52Extract Preparation
  • 02:43Footprint Extraction
  • 05:15Dephosphorylation
  • 07:07Reverse Transcription
  • 10:17Results: Ribo-seq Enables Genome-wide Quantification of Translation of Specific mRNAs Under Different Cellular Conditions
  • 11:59Conclusion

Summary

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Translational regulation plays an important role in the control of protein abundance. Here, we describe a high-throughput method for quantitative analysis of translation in the budding yeast Saccharomyces cerevisiae.

Tags

Ribosome ProfilingGenome-wideTranslationBudding YeastSaccharomyces CerevisiaeProtein TranslationTranslational RegulationCell LysatePoly(A) MRNASucrose GradientRNase ICycloheximide

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